Development and Validation of RP-HPLC Method for the determination of Linezolid in pharmaceutical dosage form
A simple, precise, accurate sensitive and specific RP-HPLC method for the determination of Linezolid in pharmaceutical dosage form. Chromatogram was run through Sunfire C18 150 x 4.6 mm, 5m. Mobile phase containing 0.01N Potassium dihydrogen phosphate: Methanol taken in the ratio 65:35 was pumped through column at a flow rate of 1.2ml/min. Buffer used in this method was 0.01N Potassium dihydrogen phosphate (4.8ph) buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 252.0nm. Retention time of Linezolid was found to be 2.469 min.