In this work, the synthesis of stable silver nanoparticles by the bioreduction method was investigated.Aqueous extract of crude black pepper (Piper nigrum) extracted at room temperature was used as reducing. UV-Vis absorption spectroscopy was used to monitor the formation of silver nanoparticles the UV–vis absorption shows a characteristicabsorption peak of silver nanoparticles at 410 nm. Thecharacteristics of the obtained silver nanoparticles were studied alsoby usingFourier transform infrared spectroscopy. The FT-IR spectra of the purified silver nanoparticles were compared withspectra ofcommercially available black pepper. X-ray diffraction analysis revealed the distinctive facets ((1 1 1),( 2 0 0), (2 2 0), and (2 2 2) planes) of silver nanoparticles.The synthesized silvernanoparticles had significant antibacterial action on both the Gram classes of bacteria namely: Staphylococcus aureus, Escherichia coli ,Pseudomonas aeruginosaand Bacillus subtilis.
