fingerprinting intra-specific diversity among coccinia grandis landraces

Research Article
Sunita Singh and Mala Parab*
DOI: 
xxx-xxxx-xxx
Subject: 
science
KeyWords: 
C. grandis, Geo-edaphic genetic differentiators, Jaccard’s similarity coefficient.
Abstract: 

Comprehension of ecological and genetic evolution underlying the populace is a prerequisite for development of effective plant conversation, breeding strategies and trace crop varieties, as without phenome and genome variance, a population cannot acclimatize to its geo-edaphic milieu. The present study set sights on morphological and genetic differentiation pattern among 30 land races of C. grandis at intra- specific level, using 18 morphological, 15 RAPD and 10 ISSR markers. The values of fruit weight, girth and texture, along leaf shape displayed the major divergence among the 18 morphological traits. In RAPD and ISSR-PCR analysis of the 561 amplified products produced, 286 bands revealed polymorphism (50.98%). A marginal higher proportion of polymorphic bands were observed using ISSR (51.38%) than RAPD (50.10%) method. Mean PIC (polymorphism information content) for each of these marker systems (0.49 for RAPD and 0.62 for ISSR) suggested that both the marker systems demonstrated significant polymorphism. Jaccard's pairwise similarity coefficients ranged from 0.06 to 0.83 (RAPD) and 0.06 to 0.81 (ISSR) respectively. Cluster analysis based on RAPD, ISSR and their combined data clearly discriminated the cultivars into different clusters. Therefore, we conclude that these markers could be successfully used to assess genetic diversity with almost equal efficiency. The information provided here would contribute to breeding program as well as evolutionary study in C. grandis