In the present study four different culture media, Czapek’s-Dox agar (CZA), Potato dextrose agar (PDA), Yeast extract sucrose agar (YESA) and Aspergillus flavus and parasitic us agar (AFPA) were used to know the efficacy for the growth of A. flavus. A total of 38 strains of A. flavus including aflatoxigenic and nonaflatoxigenic strains were analyzed for their growth response on PDA at different range of pH and temperatures. The results indicated that pH 8 and temperature 30°C were found to be optimum for the growth of A. flavus isolates in PDA medium. Further extracellular enzyme activities indicated that there was slight significant difference among tested isolates of A. flavus appeared in plate screening assay carried out for amylase, protease and lipase activity. However, the pectinase activity was same in all the tested isolates. In conclusion both aflatoxigenic and non-aflatoxigenic isolates of A. flavus did not show significant variation in extracellular enzyme activities.
