Bacteria producing poly-β-hydroxy butyrate thorough growth on renewable agribyproducts were optimized for expeditious PHB production. The conditions for optimum bacterial growth were manipulated in two isolates B2 and B3 for cost effective PHB production in the presence of organic C & N nutrient sources. Low cost renewable carbon and nitrogen sources like molasses, potato peels, mustard cake and cotton cake were incorporated to increase the PHB production and to decrease its cost in an ecofriendly process. The expedited process of PHB production had a harvest time of only 3 days. Using glucose and fructose as carbon sources and mustard cake and yeast extract as nitrogen source, two strains B2 and B3 exhibited a maximum harvestable PHB as 0.045 g/l and 0.037g/l respectively.
