Zopiclone commonly classified in category of Z-drugs. It is well known hypnotic agent used for treatment of sleep disorder. Zopiclone is non-benzodiazepine compound and its mechanism of action based on modulating benzodiazepine receptors. An isocratic reversed phase stability-indicating highperformance liquid chromatographic (HPLC) assay method was developed and validated for quantitative determination of Zopiclone in bulk drugs. Method developed with column Zorbax C18, 250 x 4.6mm 5µm or equivalent, and the mobile phase containing 0.5% glacial acetic acid. The developed method was validated with respect to linearity, accuracy (recovery), precision, system suitability, selectivity, robustness prove the stability indicating ability of the method.
