Study On Siderophore Producing Bacteria And Its Applications

Research Article
Hena.Y.Parmar and Hemalatta Chakraborty
DOI: 
xxx-xxxxx-xxxx
Subject: 
science
KeyWords: 
Toxicity, waste fried oils, Siderophore metal complex, PHB Granules, biosurfactant, biological platform.
Abstract: 

The health of biosphere is seriously affected by many factors including human activities.The major factors include toxicity caused by heavy metals in soil and aquatic life along with accumulation of plastic waste and chemical surfactants. It is observed that Bacteria are often iron limited and hence produce extracellular iron-scavenging siderophores. Unlike iron-siderophore complexes which can be taken up by species specific receptor cells, siderophore bound to other heavy metals do not enter the cell efficiently. Hence siderophore production may contribute to a heavy metal bioremediation. In the present study, siderophore was extracted from fluorescence species of Pseudomonas isolated from spinach root surface. Indication of siderophore-metal complex was determined by spectral scan and peak changes between extracted metal free siderophore and metal siderophore complexes. Apart from this, during starvation period, obtained isolate accumulatedPoly Hydroxy Butyrate (PHB) granules intracellularly in the presence of waste fried oils as a carbon source. PHB has attracted commercial interest as a new biodegradable polymer. Acceptable amount of PHB granules were obtained by growing cells in Mineral Salt Medium (MSM) with waste fried oils as a carbon source. Along with accumulating PHB granules intracellularly this bacteria also synthesize biosurfactantextracellularly as a precipitate in the same MSM medium containing carbon source. This provides an alternative to production of chemical surfactants. The best results were obtained when using waste fried olive oil as carbon source. Thus present study provided a biological platform by exploiting abilities of a single bacterium to combat major Environmental issues.