solvent-free synthesis of mannich products catalyzed by ethyl ammonium nitrate as reusable ionic liquid and their in vitro microbial studies

Research Article
Patel, H. M
Solvent-free, One pot synthesis, Mannich products, Reusable ionic liquid, UV absorbing materials Multi-component synthesis.

A solvent-free, simple one-pot reaction has been used for synthesis of mannich products (HA1-HA6) in excellent yields via three-component condensation of heterocyclic aldehyde, phenol as u.v. absorbing material and various amide derivatives using ethylammonium nitrate (EAN) as reusable ionic liquid as catalyst under 80 °C temperature. All compounds were characterized by 1H-NMR, 13C NMR and infrared spectra were recorded in KBr pellets on a Perkin–Elmer Spectrum GX FT-IR model. All compounds also screened for their in vitro antibacterial activity by using the agar dilution technique. Specially two compounds shows very good microbial activity as compared to standard drugs, HA3 and HA4 compounds showed potential activity especially against P. Aeruginosa MTCC 1688(MIC, 40-50 µg/mL), and S. Aureus MTCC 96 (MIC, 45-50 µg/mL). HA3 compound shows good Antifungal activity between 100-200 µg/mL similarly HA4shows 100-150 µg/mL.Antituberculosis activity of HA3 and HA4 compounds shows moderate activity 5-7 µg/mL using L. J. conventional method