L-Asparaginase a therapeutic protein is widely used in medical sector to diagnose and treat leukemia. It has been obtained from various sources like bacteria, fungi, yeast and plants. Microbial L-asparaginase has been found to be associated with toxicity and sensitivity due to its low specificity to asparagine. The side effects caused by microbial L-asparaginase bought medicinal plants as source into focus. In this study, L-asparaginase was screened in various medicinal plant parts in different seasons. Among all the screened plants, Phyllanthus emblica is identified as potential source for L-asparaginase production. The highest enzyme activity (20.3 IU/ml) and specific activity (5.2 IU/mg) was obtained in Phyllanthus emblica leaves during the non fruiting season. Further other medicinal plants like Aegle marmelos and Citrus nobilis contained appreciable amount of L-asparaginase. L-asparaginase from Phyllanthus emblica illustrated maximum activity at 37°C and 8.5 pH. Kinetic parameters, Km and Vmax of enzyme, were found to be 6.09mM and 88.12
