Colorectal cancer (CRC) is the third most commonly diagnosed cancer among males and the fourth most common among females worldwide. investigated the serum levels of IL-10 and TNF-α in patients with colon cancer. Fifty patients with colon cancer (21 female and 29 male) with ages ranged between (20-70) years were taken from (Al-Hussain Hospital City/Kerbala, Digestive and Liver Disease /Education Hospital Medical City Baghdad and Teaching Oncology Hospital /Baghdad Medical City/ Baghdad /Iraq).Control group consisted of 20 healthy people who were free from signs and symptoms of cancer who matched in age and gender with patients, and had no history for any gastrointestinal problem.TNF- α(TNF-α-EASIA Kit, DIAsource) and IL-10 (IL-10-EASIA Kit, DIAsource) were studied using the enzyme-linked immunosorbent assay (ELISA) method.t-test and ANOVA and Pearson correlation used to analyze results by using SPSS version 19. P-value ≤ 0.05 was considered significant.Serum CEA,Ca19-9 ,IL10 and TNF- α were increased significantly (p< 0.05) in patients compared with control group,these parameters increased no significantly (p>0.05) after chemotherapy except IL-10,so increasing of CEA,Ca19-9,IL-10,and TNFα values at stage3(10.3±3),stage1 (52±9.5),stage4(49±23)stage1(148±16) respectively no significant(p>0.05) among stages, the results revealed a strong correlation (p<0.05) between TNF and CA19.While there is no significantly correlation (p<0.05) found in the studying parameters in patients who did not treatment with chemotherapy drug. Significantly correlation of CEA,Ca19-9 ,IL10 and TNF- α with colon cancer, so increased no significantly (p>0.05) after chemotherapy except IL-10
