The aim of present work was focused on the cellulase production by Bacillus subtilis using coconut coir waste as a substrate. Coconut coir waste is an abundant natural biopolymer on earth and most dominating Agricultural waste. These agro industrial residues are cheap raw material for cellulase production. The maximum production of cellulase (in terms of glucose production) was obtained after 7 days of incubation period in solid state fermentation. The physical and nutritional parameters like pH, temperature, moisture content, and incubation period and nitrogen sources are optimized. The optimal condition for maximum biosynthesis of cellulose by B. subtitles were shown to be at pH 7.0, temperature 400 C, moisture ratio 1:1.5 (w/v), incubation period 7 days and in (NH4)2SO4 as nitrogen source.
