In this study, 2, 617 urine samples were collected from the clinically suspected patients and examined the prevalence and antifungal susceptibility pattern of Candida dubliniensis. In this study, overall prevalence of C. dubliniensis is 0.34%.The phenotypic identification methods for C. dubliniensis included germ tube test, production of rough colonies and chlamydospores, determination of assimilation and fermentation, HiCandida identification kit, morphology on Hichrome agar and growth at 45°C. Totally, 112 isolates were recovered from the urine samples and those were directly subjected to germ tube test. Out of which, 61 (54.46%) isolates were positive in germ tube production. Among 61 germ tube positive isolates, 9 were identified as C. dubliniensis with the prevalence of 14.75%. Antifungal susceptibility test was performed with amphothericin B, clotrimazole, fluconazole, itraconazole, ketoconazole, miconazole and nystatin. All the 9 isolates showed (100%) sensitive to amphotericin B, followed by fluconazole and ketoconazole (88.88%). This study highlighted the usefulness of Hichrome agar and temperature test (growth at 45°C) in presumptive identification of C. dubliniensis and combats the existing view that this species occur in particular anatomic sites of specific population through this species recovered from urine of HIV/AIDS negative individuals.
