Isolation, Identification and Molecular Characterization of Actinomycete Strain from the Laterite Soil of Kandukuru

Research Article
B. Chinna Narasaiah, *V. Leelavathi, G. Sudhakar and P. Mariyadasu
DOI: 
xxx-xxxx-xxxx
Subject: 
Science
KeyWords: 
Actinomycetes, Phylogenetic tree, Scanning Electron Microscopy, GenBank
Abstract: 

The laterite soil was collected from the Kandukuru of Prakasahm District and analyzed for its physico-chemical properties and pretreated and used for isolation of actinomycetes. The isolated strain was designated as CN-4.The morphological charecterisation carried with genomic (16 rDNA gene sequencing) analysis. The amplified and sequenced 16S rDNA was compared with the sequences in GenBank using BLAST and aligned with the sequences retrieved from NCBI GenBank database using the Clustal W method. The phylogenetic tree was constructed based on Maximum Likelihood method using bootstrap analysis and micro morphology of the strain through Scanning Electron Microscopy (SEM) was also carried out. Based on the scanning electron microscopic observation and phylogenetic tree construction the isolated strain from the laterite soil of Kandukuru was identified as Streptomyces albus CN-4.