The thermoalkalophilic Bacillus sp.JB-99 was employed in this study to immobilize it in sodium alginate and produce alkaline protease using chemically defined medium. The cell concentration/ml/gm of beads was 6x106 cfu. The parameters optimized for the production of alkaline protease are; 25g/l sodium alginate, 300 beads/flask, 8.5-9.0 medium pH, 72h incubation period yielded a maximum of 3700 U/ml and ends up with 120h yielded 3800U/ml. This was about 3 fold lower than that obtained by free cells (12000 U/ml). The cell leakage was almost proportionate with initial cell loading and time of incubation. The repeated batch cultures of cells have shown viability till 6 cycles. At every 48h intervals upto 120h shown gradual increase in enzyme production till 6th cycle with a max of 4000U/ml.
