identification, disease transmission and phytopathological effects of bacterial pathogens associated with vicia faba

Research Article
Manju Meena* and Ashwani Kumar Verma
DOI: 
http://dx.doi.org/10.24327/ijrsr.2021.1312.0508
Subject: 
science
KeyWords: 
Vicia faba, Pseudomonas aeruginosa, Pseudomonas syringae, Pseudomonasviridiflava, Disease transmission.
Abstract: 

Symptomatic infected Vicia faba L. (Fava bean) plant parts were collected from three districts (Alwar, Bundi, and Kota) of Rajasthan to study the presence of bacterial pathogens. A total of 11 pathogenic bacteria isolated were on the basis of morphological studies such as color, shape and opacity. In biochemical analysis, out of 11 isolates, 2 bacterial isolates showed gram negative, rod shapes, catalase positive, KOH positive, oxidase positive, indole production negative, citrate utilization positive, cetrimide positive, gelatin hydrolysis positive and arginine dihydrolase positive results of Pseudomonas aeruginosa, 8 isolates showed gram negative, rod shapes, catalase positive, KOH positive, oxidase negative, indole production negative, citrate utilization negative, cetrimide negative, carbohydrate fermentation positive results of Pseudomonas syringae and 1 isolates gave gram negative, rod shapes, catalase positive, KOH positive, oxidase negative, indole production negative, citrate utilization negative, cetrimide negative, carbohydrate fermentation negative results of Pseudomonas viridiflava.The shriveling, rotting, white bacterial growth on and around seedborne inoculums and less germination rate was observed in disease transmission experiment. Additionally same pathogens were detected in transmission methods that revealed the transmission of bacteria from one generation to other by vertical transmission. Artificially infected seeds in phytopathogenecity test also caused the reduction in germination, reduced shoot-root lengths and blacking of radicle.