Porous materials and nanoparticles are now day on the scope of many researches that study different application in all science areas, and the medical field is impacted by this technology. Applications like drug release, sensors, cancer treatments, tissue engineering, implants, etc. On this development the silicone or polydimethylsiloxane (PDMS) play a key role, this polymer is frequently used for medical applications as cardiac pacemakers, mammary prostheses, implants, heart valves, etc. Porous silicone materials with immobilize nanoparticles can resolve some limitations from the nonporous silicone. At leaching method with sieve sugar was used to prepare a porous silicone with a controlled pore size distribution and mean pore size of 160 micro meters. Later this material was used to immobilized gold, silver and copper nanoparticles, cytotoxic study with MTT assay was running through ISO/EN 10993-5 and ISO/EN 10993-12, and the study non evidence statistical cytotoxicity differences between pure porous silicone matrix and porous silicone matrix with silver, gold nanoparticles or their combination, but copper nanoparticles immobilized over the silicon porous matrix evidence statistical difference on cytotoxic effect over the same cells (L929 fibroblast).
