genotoxicity in a freshwater fish, cirrhinus mrigala exposed to dyeing industry effluent by using micronucleus test

Research Article
Harbhajan Kaur, Rohit Kalotra, Gurinder K Walia* and Diana Handa
DOI: 
xxx-xxxx-xxx
Subject: 
science
KeyWords: 
Piscine micronucleus test, aquatic pollution, dyeing industry effluent, Cirrhinus mrigala
Abstract: 

The micronucleus test has been used to detect the genotoxic effect of mutagens in environment. Genotoxic effects of dyeing industry effluent have been evaluated by using piscine micronucleus test. In the present study, 96h LC50 of dyeing industry effluent against Cirrhinus mrigala came out to be 48.97%. Three sublethal concentrations (24.48%, 12.24% and 6.12%) were made and fishes were exposed to these concentrations. Blood from anterior kidney was used to study micronuclei in erythrocytes of fishes after 24h, 48h, 72h and 96h. There were significant differences (p<0.05) between the control and treated fishes. The results showed that at higher concentration (24.48%) and exposure time (96h), there was maximum number of micronuclei in erythrocytes as compared to other concentrations at various time durations as well as in control.