Estimation Of Amines And Amine Forming Bacterias In Edible Marine Fish Sardinella Longiceps And Its Product

Research Article
Merline X., Chitra G and Dhanalakshmi B
DOI: 
xxx-xxxxx-xxxx
Subject: 
science
KeyWords: 
Sardinella longiceps, Biogenic amines, Histamine, E.coli.
Abstract: 

Biogenic amines are formed as a result of amino acid decarboxylation and are linked to food deterioration. Analysis of these metabolites may be great importance to determine food quality. The aim of the study is to estimate the biogenic amines and amine forming bacterias in edible marine fish Sardinella longiceps and its product. The fish samples were collected from landing center in Tuticorin coastal area, south east India and from local fish market, Ukkadam, Coimbatore. The dry fish were procured from retail shop in Coimbatore. From the result obtained the samples collected from the market showed the highest total viable count 76×105 cfu/g, then landing center and dry product which showed the lowest total viable count 1.0×105 cfu/g, 0.7×105 cfu/g respectively, which is below the permissible limit of 7 ×105 cfu/g. In this study the amine forming bacteria were isolated from the sample collected from the local market. Different bacterias were isolated from the market sample and the isolated bacterias are E.coli, Pseudomonas, Klebsiella, Proteus mirabilis, Shigella dysenteriae, Staphylococcus aureus, Streptococcus faecalis, Salmonella typhimurium, and Enterobacter aerogens. In dry product E.coli and Staphylococcus aureus were isolated. In the present study the concentration of four biogenic amines; Histamine, putrescine, tyramine and cadaverine in samples collected from market fish was determined, from the revealed results the concentration of histamine (11.004mg/ kg) was higher than the other three biogenic amines, cadaverine, tyramine, putrescine (7.586, 7.120, and 3.495mg/kg) respectively.