In the present investigation the bioremediation of hydrocarbon in contaminated soils by the cultures of hydrocarbon-degrading bacteria was investigated. Petrol and diesel oil are non degradable. In our study we have identified the Bacillus and Pseudomonas spp as a great potential for hydrocarbon degradation. The Bacillus and Pseudomonas spp. were isolated from hydrocarbon contaminated soil and various morphological and biochemical test were applied for their identification. In optimization studies, the best results observed for Bacillus and Pseudomonas spp .were 2T engine oil as the suitable carbon source, Optimum pH and temperature is 7 and 37°C respectively. The present study reveals the fact that Bacillus and Pseudomonas spp. have high potential for hydrocarbon degradation and can be used especially for microbial enhanced oil recovery and bioremediation of hydrocarbons in near future.
