cardio protective effect of nelumbo nucifera flower extract against isoproterenol induced oxidative stress in male swiss albino rats

Research Article
Kirithika.T., Gomathi, R and Usha, K
Isoproterenol, Intraperitoneal, cardiac enzymes, antioxidants

Nelumbo nucifera Gaertn (Family- Nymphaeaceae) is a well-known plant in ancient medical sciences. The leaf of Nelumbo nucifera Gaertn (family Nymphaeaceae) has been used for summer heat syndrome as home remedy in Japan and China, and it has recently been used to treat obesity in China. So the present study investigate the cardioprotective effect of Nelumbo nucifera flowers in isoproterenol induced rats. Cardiomyopathy was induced by a subcutaneous administration of isoproterenol. The positive hypertrophy response of isoproterenol caused a severe oxidative stress in the myocardium through increased lipid per oxidation. Nelumbo nucifera was administered intraperitoneally at a dose of 200 mg/kg for a period of 30 days. On 29th and 30th days the rats were induced with isoproterenol (20 mg/100g subcutaneously twice at an interval of 24 hours). At the end of experimental period, on day 29 th, 48 hour after injection of ISO administration, the animals were sacrificed under an overdose of anaesthesia. Blood was collected and was used for the determination of diagnostic cardiac marker enzyme and the heart was excised immediately, cleaned free of extraneous material and perfused with ice cold saline which are used for enzymic and non enzymic antioxidant estimations and also stored in 10% formalin, which are used for Histopathological studies